Your EXPERT for animal genetics

Contact Info

ISAG 2020
Standard
ISAG 2006
230 SNPs
Marker
22 STRs
14 – 21 business days
Test duration
7 – 14 business days
EDTA blood or special swab (eNAT)
Sample material
EDTA blood or special swab (eNAT) or dry cheek swab

Premium (SNP)

Standard ISAG 2020
Marker 230 SNPs
Sample material EDTA blood or special smear (eNAT)
Test duration 14-21 working days

Classic (STR)

Standard ISAG 2006
Marker 22 STRs
Sample material EDTA blood or special swab (eNAT) or dry cheek swab
Test duration 7-14 working days

FAQs DNA profile DOG

What is a DNA profile?

A DNA profile is the genetic fingerprint of an animal. The DNA profile is unique for each animal, independent of age and unchangeable. This profile can be used to identify an animal independently of the chip.

Why do I need a DNA profile?

A DNA profile is often required by breeding associations for the registration of animals. It is also the basis for parentage checks. The DNA profile is also used for the breed determination test. If the chip is lost or can no longer be read, this profile can be used to identify your pet beyond doubt.

What material is needed to create a DNA profile?

We require either 0.5 to 1.0 ml of EDTA blood or two dry cheek swabs from the cheek mucosa of the animal (Classic DNA profile) or a special swab (Premium DNA profile).

Can I take the swab for a DNA profile myself?

In principle, a swab test can be carried out by the pet owner himself. However, many breeding organisations insist that the sample is taken by an independent person (vet or breed warden) who verifies the identity of the animal beyond doubt with the chip number or stud book number.

What should I look out for when taking a cheek swab?

Your dog should be separated from other animals for one to two hours before a swab is taken and should not eat during this time. Otherwise, foreign genetic material could be present in the oral cavity of the animal being tested as a result of suckling by the mother or playing with other dogs. Such a contaminated sample could falsify the result. You can find instructions for the collection here.

How do I take a cheek swab?
When will I receive a digital certificate for my DNA profile?

A digital certificate for the DNA profile is always created automatically free of charge if the sample has been taken by an independent person (vet or breed warden) and the identity of the animal has been confirmed by signature, stating the chip number or studbook number. Postal certificates can be ordered, but are subject to a fee.

What does the DNA profile tell me?

The Classic DNA profile determines the lengths of defined microsatellite markers established by the International Society for Animal Genetics (ISAG 2006). For the premium DNA profile, 230 SNPs (Short Nucleotide Polymorphisms) are determined, which were also defined by the ISAG (ISAG 2020). The combination of values on the individual markers is unique for an animal and was passed on from mother and father to the offspring. The DNA profile does not provide any further information on hereditary diseases, coat colors or characteristics of the animal.

What is the difference between a Classic and a Premium DNA profile?

In both forms of DNA profiling, a genetic fingerprint is created. The Classic DNA profile (ISAG 2006) is based on identification using microsatellite markers, while the Premium DNA profile (ISAG 2020) is based on identification using short nucleotide polymorphisms (SNPs).

Please note that Classic and Premium DNA profiles are not comparable with each other. For parentage analyses, the offspring and parents must all have the same DNA profiles, either Classic or Premium DNA profiles.

Which DNA profile do I need? Classic or Premium DNA profile?

Please contact your breeding club if you are unsure which DNA profile you need. Typically, a club will have opted for a DNA profile according to the ISAG 2006 (Classic DNA profile) or ISAG 2020 (Premium DNA profile) standards.

What do I need for a parentage analysis?

For a parentage analysis we need DNA profiles of the same type (Classic or Premium DNA profile) from mother, father and offspring. If a DNA profile of the parent animals is already available, a copy of this can be submitted. If you yourself were the invoice recipient of the DNA profile of the parent animals, it is sufficient to state the LABOKLIN report number.

Can I submit DNA profiles for parentage analysis?

Yes, customers can submit DNA profiles (also from other laboratories) for parentage tests together with their samples as copies or files by e-mail.

Please ensure that the DNA profiles of offspring and parents all comply with either ISAG 2006 or ISAG 2020 standards to ensure comparability within a case.

Can I carry out a parentage analysis with only one parent?

If only one parent is available and a reliable result is desired, we recommend the Premium DNA profile together with a biostatistical calculation for the parentage analysis.

A comparison with only one parent animal using the Classic DNA profile can also be carried out, but does not always provide an unequivocal result.

If we find deviations in the DNA profile of the parent animal to the offspring, paternity or maternity can be ruled out. This result is certain.

If we find no deviations, paternity or maternity cannot be ruled out. This means that the animal is a possible father or mother, but it could also be an animal whose DNA profile does by chance not differ from the DNA profile of the puppy. We see such cases when the animals are related to each other or when they are from a breed with a small population.

My bitch has several puppies and several fathers are possible. Do I have to carry out the parentage analysis for all puppies?

As dogs can have different fathers in a litter after multiple mating, it is essential to check each puppy against each potential father for parentage verification. This is the only way to ensure that each puppy is assigned the correct sire. The result of one puppy cannot automatically be transferred to all puppies in a litter.

How reliable is the parentage analysis?

If both parents are present, the exclusion or qualification of parent animals can be correctly determined with a probability of over 99.9% with both the Classic and the Premium DNA profile. If only one parent is present, the exclusion of the animal can be reliably determined with both the Classic and the Premium DNA profile. The statement as to whether an animal is the mother or father of the tested offspring can only be confirmed with a high degree of probability using the Premium DNA profile together with a biostatistical analysis.